micro-computed tomography, confocal microscopy and volume electron microscopy. Stereology is, therefore, perfectly suited to be combined with 3D imaging techniques which cover a wide range of complementary sample sizes and resolutions, e.g. Thus, three-dimensionality is a built-in property of stereological sampling and measurement tools.
It takes into account the fact that, in traditional microscopy such as conventional light and transmission electron microscopy, although one has to rely on measurements on nearly two-dimensional sections from fixed and embedded tissue samples, the quantitative data obtained by these measurements should characterize the real three-dimensional properties of the biological objects and not just their “flatland” appearance on the sections. Stereology is the method of choice for the quantitative assessment of biological objects in microscopy.
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